AtaGenix Laboratories

Home - Support Center - Case Studies - AbPro™ Antibody

Antibody Gene Sequencing and Validation Case Study | AtaGenix Custom Antibody Development

Release time: 2024-11-05   View volume: 652

 

Figure 1: Schematic of Antibody Coding Gene Structure

Figure 1: Schematic of antibody coding gene structure, including 5’ and 3’ untranslated regions (UTRs). This illustrates the framework of heavy and light chain organization.

Figure 2: PCR Amplification of Heavy and Light Chain Variable Regions

Figure 2: PCR amplification of antibody heavy (VH) and light chain (VL) variable regions, showing clear amplification bands, confirming the successful cloning of variable regions for sequencing.

Figure 3: Colony PCR Validation Gel

Figure 3: Colony PCR validation gel. M: DL10000 DNA Marker. The clear in-frame amplification bands confirm successful insertion of VH and VL sequences into the cloning vector.

Sequence V-GENE and Allele Functionality V-REGION Identity (%) J-GENE and Allele D-GENE and Allele AA Junction Junction Frame
VH Musmus IGHV1-71-16*01 F Productive 96.53% Musmus IGHJ4*01 F Musmus IGHD2-4*01 F CAITTGYAMDYW In-frame
VL Musmus IGKV4-74*01 F Productive 97.51% Musmus IGKJ4*01 F - CHQYYRSPFTF In-frame
Figure 4: Antibody Heavy and Light Chain Sequence Analysis

Figure 4: Antibody heavy and light chain sequence analysis. High sequence identity (>96%) confirms accurate cloning and alignment of VH and VL regions.

Figure 5: Antibody Heavy and Light Chain Sequencing Peak Map

Figure 5: Sequencing peak map of antibody VH and VL chains. Clear and consistent peaks demonstrate sequencing accuracy and reliable antibody variable region identification.

Need expert support for antibody cloning, sequencing, and validation? AtaGenix delivers end-to-end custom antibody services—from design to QC.

Messages