Antibody Gene Sequencing and Validation Case Study | AtaGenix Custom Antibody Development

Project Snapshot — End-to-end antibody gene sequencing and validation case: PCR amplification of VH/VL variable regions, colony PCR confirmation, Sanger sequencing with >96% germline identity, and in-frame junction analysis — demonstrating AtaGenix’s complete antibody cloning and QC workflow.

Figure 1: Schematic of antibody coding gene structure, including 5’ and 3’ untranslated regions (UTRs), illustrating heavy and light chain organization.

Figure 2: PCR amplification of antibody heavy (VH) and light chain (VL) variable regions, confirming successful cloning for downstream sequencing.

Figure 3: Colony PCR validation gel. M: DL10000 DNA Marker. In-frame amplification bands confirm successful insertion of VH and VL sequences into the cloning vector.

Figure 4: Antibody heavy and light chain sequence analysis. High germline identity (>96%) confirms accurate cloning and alignment of VH and VL regions.

Figure 5: Sanger sequencing chromatogram of antibody VH and VL chains. Clean, non-overlapping peaks confirm sequencing accuracy and reliable variable region identification.

Need expert support for antibody cloning, sequencing, and validation? AtaGenix delivers end-to-end custom antibody services — from design to QC.

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