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Bacillus

B. subtilis Protein Expression Services

Experience efficient, endotoxin-free, secreted protein production with AtaGenix's B. subtilis expression system. Our expert team delivers high-quality proteins for research, industrial, and therapeutic applications.

Why Choose B. subtilis?

Endotoxin-Free Production: Ideal for therapeutic and diagnostic applications requiring high purity.
Efficient Secretion: Proteins are secreted directly into the culture medium, simplifying purification and boosting recovery.
GRAS Status: Recognized as safe by regulatory bodies, applicable to diverse use cases.
High Yield & Purity: For specific targets, B. subtilis can outperform E. coli in secretion and downstream purity.

Our B. subtilis Expression Process

1. Gene Synthesis & Optimization
Custom gene design and codon optimization for B. subtilis to maximize expression efficiency.

2. Vector Construction
Subcloning into high-expression vectors tailored for B. subtilis secretion and stability.

3. Strain Transformation
Transformation into multiple B. subtilis strains and host selection based on expression and secretion.

4. Expression Optimization
Fine-tuning temperature, induction strategy, media formulation, and culture parameters for optimal yield.

5. Purification & Analysis
Chromatography-based purification and comprehensive analysis (SDS-PAGE, Western Blot, concentration/quantification).

Unique Advantages of AtaGenix

Extensive Experience: 1,500+ proteins expressed across systems, including B. subtilis.
Expert Technical Team: Dedicated specialists provide end-to-end guidance and tailored solutions.
Integrated Solutions: Seamless service from gene synthesis to production and QC—shorter timelines, lower risk.
Advanced Analytics: Rigorous QC ensures purity, integrity, and activity (SDS-PAGE, WB, quantification).
Customized Services: Expression and purification are tuned to your target’s biology and downstream use.
Scalability: From pilot studies to larger-scale production with consistent performance.
High-Throughput Screening: For difficult proteins, we screen conditions/hosts to unlock expression.
CRO Reliability: Proven delivery and documentation aligned to research and regulatory expectations.

Advanced purification techniques and comprehensive analysis (SDS-PAGE, Western Blot, quantification).

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