AtaGenix Laboratories

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Bacillus

B. subtilis Protein Expression Services

Experience efficient, endotoxin-free protein production with AtaGenix's B. subtilis expression system. Our expert team delivers high-quality proteins for research, industrial, and therapeutic applications.

Why Choose B. subtilis?

Endotoxin-Free Production: Ideal for therapeutic and diagnostic applications requiring high purity.
Efficient Secretion: Proteins are secreted directly into the culture medium, simplifying purification.
GRAS Certified: Recognized as safe by regulatory bodies, suitable for various applications.
High Yield and Purity: Achieves higher expression yields and purity for certain proteins compared to E. coli.

Our B. subtilis Expression Process

1. Gene Synthesis & Optimization
Custom gene design and codon optimization for B. subtilis to maximize expression efficiency.

2. Vector Construction
Subcloning into high-expression vectors tailored for B. subtilis.

3. Strain Transformation
Transformation into multiple B. subtilis strains to select the best host.

4. Expression Optimization
Fine-tuning of temperature, induction, and media for optimal protein yield.

5. Purification & Analysis
Advanced purification techniques and comprehensive analysis (SDS-PAGE, Western Blot, quantification).

Unique Advantages of AtaGenix

Extensive Experience: Successfully expressed over 1500 proteins across various systems, including B. subtilis.
Expert Technical Team: Our experienced and professional team provides dedicated support and personalized assistance throughout your project.
Integrated Solutions: Seamless service from gene synthesis to protein production, reducing complexity and time.
Advanced Analytics: Rigorous quality control and advanced analysis ensure the highest protein purity and functionality.
Customized Services: Tailored expression and purification processes to meet your specific needs.
Scalability: From small-scale research to large-scale production, we adapt to your project requirements.
Expert Support: Our protein expression specialists are committed to your project's success, offering insights and solutions at every stage.
High-Throughput Screening: For challenging proteins, we offer high-throughput screening to identify the best expression conditions.

Advanced purification techniques and comprehensive analysis (SDS-PAGE, Western Blot, quantification).

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