AtaGenix Laboratories

Home - Mammalian

HEK293 Transient

AtaGenix offers advanced mammalian protein expression services leveraging CHO and HEK293 cell systems. By integrating cutting-edge transient transfection platforms such as XtenCHO and FreeStyle CHO, we ensure near-native folding, proper glycosylation, and high-yield production of recombinant proteins. Our end-to-end workflow supports antigen preparation, antibody discovery, and biopharmaceutical development, providing research teams with consistent, scalable, and regulatory-ready protein solutions.

Contact Us

AtaGenix delivers a fully integrated CHO and HEK293 expression platform, combining proprietary culture media, advanced transfection reagents, and optimized purification workflows such as ion exchange chromatography and gel filtration. This system ensures high-yield recombinant protein and antibody production with two major advantages:

  • Precision Control: Tailored expression and purification protocols for consistent quality and reproducibility.
  • Cost Efficiency: Streamlined processes that reduce production costs while maintaining high performance.
AtaGenix CHO/HEK293 Protein & Antibody Expression Workflow 1. Gene Design & Vector Construction Sequence Optimization Signal peptide / Codon / Glycosylation Gene Synthesis PCR / Cloning Vector Construction Expression plasmid / Tags 2. Expression (CHO / HEK293) Transient Expression XtenCHO / FreeStyle Stable Expression Pools / Cell Lines Culture Optimization Feeding / Temp / pH Scale-Up Production Shake Flask → 5–20L → 200L 3. Purification & Quality Control Capture Protein A/G / Ni-NTA Polishing IEX / HIC / SEC Buffer Exchange / Concentration UF / Dialysis / Formulation Quality Control SDS-PAGE / SEC-HPLC / Endotoxin Deliverable: ≥95% Purity + Report   Note: Dashed arrows represent optional routes (e.g., direct scale-up)

HEK293 Transient FAQs

More Featured Antibody Services

Featured Services

Ask Us