How to Choose the Right Protein Tag??
This article systematically reviews the characteristics, advantages, disadvantages, and applications of commonly used protein tags such as His, GST, Flag, MBP, HA, Strep, and SUMO. Drawing on practical experience in recombinant protein expression and purification, it analyzes the suitability of each tag in different expression systems and highlights key considerations. The article offers practical tag selection and combination strategies to help researchers optimize experimental design and enhance the efficiency and precision of protein expression and purification.
