AtaGenix Laboratories
AtaGenix Laboratories
AtaGenix provides custom polyclonal antibody production from antigen design through serum purification and QC-validated delivery. Polyclonal antibodies recognize multiple epitopes on the target antigen, delivering robust signal across ELISA, Western Blot, IHC, IF, and immunoprecipitation applications. Full service delivery in 6–8 weeks, with purification completed within 7 days of final bleed.
6+
Host Species
6–8 wks
Total Timeline
7 days
Blood to Purified Ab
5 Systems
For Antigen Preparation
Available host species: rabbit, mouse, rat, guinea pig, sheep, and chicken. Rabbit is the default for most research applications; chicken (IgY) is recommended when you need antibodies that do not cross-react with mammalian IgG or bind Fc receptors — ideal for multi-label experiments and reducing background in mammalian tissue samples.
| Advantage | Why It Matters |
| No IgG cross-reactivity | IgY and IgG have no homologous cross-reaction — perfect for multi-species co-staining experiments |
| No Fc receptor binding | IgY lacks the Fc region that binds mammalian Fc receptors, dramatically reducing non-specific background |
| Non-invasive collection | Antibody purified from egg yolks, not blood — higher yield per animal, lower cost, continuous production |
| Strong response to conserved mammalian proteins | Evolutionary distance between birds and mammals triggers stronger immune response to conserved epitopes |
Antigen Expertise Built In
Five expression systems (mammalian, E. coli, insect, yeast, B. subtilis) for recombinant antigen preparation. Peptide synthesis and small molecule/hapten conjugation (KLH, BSA, OVA) handled in-house. No need to source antigens separately.
7-Day Purification Turnaround
From final bleed to QC-passed, purified polyclonal antibody in just 7 days. Protein A/G affinity purification with antigen-specific affinity purification available for maximum specificity.
Full Downstream Validation
ELISA titer confirmation, Western Blot, and IF/IHC validation available. Serum samples provided at interim bleeds so you can track immune response before final collection.
AtaGenix's polyclonal antibody service covers the complete pipeline from antigen design through immunization, serum collection, purification, and QC validation. Antigens can be prepared in-house using any of our five expression systems, or clients can provide their own. Available hosts: rabbit, mouse, rat, guinea pig, sheep, and chicken (IgY). Optimized immunization protocols with 3–5 subcutaneous injections and interim serum monitoring. Purification via Protein A/G affinity chromatography, with antigen-specific affinity purification as an option. Total timeline: 6–8 weeks; purification turnaround 7 days from final bleed.
A 5-stage pipeline from antigen design to purified, QC-validated polyclonal antibody. Total timeline: 6–8 weeks.
01
Antigen Design
Custom epitope selection
Peptide synthesis or protein expression
Carrier conjugation (KLH/BSA)
02
Antigen Production
Recombinant expression & purification
or peptide synthesis with modifications
QC validation of antigen integrity
03
Immunization
Host species selection
3–5 subcutaneous injections
Interim bleed & ELISA titer monitoring
04
Antibody Testing
ELISA & Western Blot screening
Affinity & specificity assessment
Serum sample provided for client review
05
Purification & QC
Protein A/G affinity purification
Optional antigen-specific purification
7-day turnaround from bleed to delivery
Service Scope
| ✓ Custom antigen design & epitope selection | ✓ Recombinant protein or peptide antigen prep |
| ✓ Carrier conjugation (KLH, BSA, OVA) | ✓ Small molecule / hapten conjugation |
| ✓ Hosts: rabbit, mouse, rat, guinea pig, sheep, chicken | ✓ Optimized immunization (3–5 injections) |
| ✓ Interim bleed + ELISA titer monitoring | ✓ Protein A/G or antigen-specific purification |
| ✓ QC: ELISA, WB, IF/IHC (optional) | ✓ Deliverables: serum + purified Ab + report |
| Species | Best For | Antibody Type |
| Rabbit | Most versatile; high affinity, IHC/IF/WB compatible | IgG |
| Chicken | Multi-label experiments; low background on mammalian tissue; conserved protein targets | IgY (from egg yolks) |
| Mouse / Rat | When downstream applications require rodent-origin antibodies | IgG |
| Guinea Pig | Strong immune response; good for co-staining with mouse/rabbit mAbs | IgG |
| Sheep | Large serum volume; high total antibody yield per animal | IgG |
Need monoclonal instead? Explore Mouse Hybridoma for traditional mouse mAb, Rabbit Single B Cell for high-affinity rabbit mAb, or Phage Display for fully human antibodies. For PTM-specific antibodies (phospho, methyl, acetyl), see our dedicated Modification Antibody Service.
Which host species should I choose?
Rabbit is the default recommendation for most applications — it produces high-affinity antibodies compatible with the broadest range of secondary antibodies and detection systems. Choose chicken (IgY) when your experiment involves multi-species co-staining or when you need to minimize background on mammalian tissues (IgY does not bind mammalian Fc receptors). Choose sheep when you need large serum volumes. Our team will recommend the optimal species based on your target and application.
Can I provide my own antigen?
Yes. You can provide purified protein (≥85% purity recommended, ≥3 mg total: 2.5 mg for immunization + 0.5 mg for ELISA screening), synthetic peptide, or even bacterial/viral samples. Alternatively, AtaGenix can design and prepare the antigen using any of our five expression systems or peptide synthesis platform.
How long does it take and what do I get?
6–8 weeks from antigen to delivered antibody. Deliverables include: unpurified serum sample (for your own testing), all Protein A/G-purified polyclonal antibody, and a detailed technical report with ELISA titer data and purification QC. From final bleed to purified antibody delivery takes just 7 days.
What is the difference between polyclonal and monoclonal antibodies?
Polyclonal antibodies are a mixture of antibodies recognizing multiple epitopes on the target, providing strong signal and broad reactivity — ideal for detection (WB, IHC, IF, ELISA). Monoclonal antibodies recognize a single epitope with high specificity — preferred for quantitative assays, therapeutic development, and applications requiring batch-to-batch consistency. AtaGenix offers both; polyclonal is faster (6–8 weeks) and lower cost, while monoclonal requires 16–20+ weeks but delivers reproducible, sequence-defined reagents.
Antigen quantity requirements: protein ≥3 mg (≥85% purity) or peptide ≥5 mg. Immune response and antibody titer are target-dependent. Cross-link URLs should be confirmed with actual page IDs. Quote-based pricing.
Contact Us
+86-27-6552-3339
info@atagenix.com
Building C, R & D Building, No. 666, Shendun 4th Road, Donghu New Technology Development Zone, Wuhan
