Protein Services

AtaGenix's recombinant protein expression platform provides custom production across six optimized expression systems — from gene synthesis to bioreactor-scale delivery. Each system is selected to match your protein's specific requirements for glycosylation, folding, yield, endotoxin, and downstream application.

Expression Systems

200L

Scale-Up Capacity

XtenCHO™

Proprietary Mammalian System

Gene→Protein

End-to-End Service

Every project includes gene synthesis, codon optimization, vector construction, expression, purification, and QC (SDS-PAGE + technical report). Not sure which system to choose? Use the comparison table below or consult our team — we recommend the optimal host based on your protein type, PTM requirements, scale, and application.

6-System Comparison: Choose Your Expression Host

System	Best For	PTMs	Timeline	Endotoxin
Mammalian (CHO/HEK)	Therapeutic antibodies, Fc-fusions, receptor ectodomains, viral antigens	Complex glycosylation, disulfide bonds	~2 wks (transient)	Low
E. coli	Small proteins, His-tag proteins, enzymes, antigens for immunization, structural biology	None (no glycosylation)	1–2 wks	High (removable)
Yeast (Pichia)	Secreted proteins, industrial enzymes, high-mannose glycoproteins, scalable fermentation	High-mannose glycosylation	6–10 wks	Low
Insect / BEVS	Multi-subunit complexes, VLPs, kinases, glycoproteins requiring simple eukaryotic processing	Simple glycosylation, phosphorylation	6–8 wks	Low
B. subtilis	Endotoxin-free proteins, GRAS-status products, secreted enzymes, food/feed applications	None	7–12 wks	Zero
MHC I Complex	Peptide-MHC tetramers, HLA complexes, T-cell detection reagents, neoantigen screening	HEK293-based native folding	Consult	Low

Not Sure Which System? Start Here

“I need an antibody or Fc-fusion”

→ Mammalian (CHO/HEK) — correct glycosylation and disulfide bonds required. CHO for manufacturing-representative glycans; HEK293 for human-type sialylation.

“I need the cheapest / fastest option”

→ E. coli — lowest cost, 1–2 week turnaround, highest yield per liter. Best for small non-glycosylated proteins, antigens, and structural biology.

“I need endotoxin-free protein”

→ B. subtilis — zero endotoxin by nature (Gram-positive), GRAS-status, secreted expression. No endotoxin removal step needed.

“I need a VLP or multi-subunit complex”

→ Insect / Baculovirus (BEVS) — co-expression of multiple subunits via Bac-to-Bac system. Sf9, Hi5, S2 host cells available.

“I need scalable secreted production”

→ Yeast (Pichia pastoris) — methanol-inducible AOX1 promoter, high cell density fermentation, secreted into media for easy purification.

“I need pMHC tetramers”

→ MHC I Complex Preparation — HEK293-based co-expression, peptide loading, tetramerization, SPR+FACS QC. 260+ MHC/HLA reagents in abinScience catalog.