AtaGenix Laboratories

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Scale ups

Consistent, Scalable, and Rapid Antibody Manufacturing for Research and Preclinical Development

Overview

Producing gram-level quantities of recombinant antibodies demands more than just capacity—it requires robust process control, scalability, and lot-to-lot consistency. At AtaGenix, we offer large-scale antibody production services tailored to research, diagnostic, and preclinical needs. Whether you require milligram-scale pilot runs or multi-gram GMP-like production, our platform delivers high-yield, high-purity antibodies with short turnaround times.

With over 14 years of experience in antibody expression and purification, AtaGenix supports industrial-scale manufacturing through optimized transient expression in mammalian cells, as well as stable cell pool or stable cell line-based production.

Service Highlights

✅ Milligram to gram-level scale-up (10 mg – 10 g)
✅ Transient expression and stable pool/line options
✅ CHO and HEK293 cell systems
✅ Endotoxin < 0.1 EU/mg, purity >95%
✅ 2–4 week standard turnaround
✅ Process transparency and project milestone tracking

Production Formats

We support a wide range of antibody formats and species:

Full-length IgG (mouse, rabbit, human, camelid)
scFv, Fab, VHH/nanobody
Bispecific formats (CrossMAb, BiTE, IgG-scFv)
Fc-fusion proteins and therapeutic scaffolds

Workflow & Timeline

Stage Description Timeline
1. Gene Synthesis & Cloning Codon optimization, vector design, plasmid amplification ~1 week
2. Pilot Expression (Optional) Transient small-scale transfection to verify expression and quality ~1 week
3. Scale-Up Production Up to 200L transient transfection or stable pool fermentation 2–3 weeks
4. Purification & QC Protein A/G purification, endotoxin removal, SDS-PAGE, SEC-HPLC 3–5 days

Quality Control

All large-scale antibody production projects undergo strict quality validation:

SDS-PAGE and Coomassie staining (>95% purity)
Endotoxin testing (LAL assay)
SEC-HPLC for aggregation profile
BCA or UV quantification
Optional: Biacore/Octet for binding confirmation

Optional Add-Ons

Protein characterization (KD by SPR/BLI)
Functional validation (ELISA, FACS, neutralization assay)
Buffer exchange, concentration, lyophilization
Aliquoting and custom labeling
Stability studies (thermal, freeze-thaw, storage)

Case Example

Mouse IgG2a Production at 2g+ Scale

Expression: transient CHO, 200L batch
Yield: 288 mg/L, Total: 1.97g
Purity: SDS-PAGE >95%, SEC-HPLC >99%
Turnaround: 4 weeks

Why Choose AtaGenix?

✅ Experienced project team with a proven track record in large-scale manufacturing
⚙️ End-to-end in-house capacity from DNA to purified antibody
✅ Flexible expression systems for different formats and timelines
⚙️ Transparent process management and milestone updates
⚙️Global-ready delivery with export documentation and cold-chain logistics

Whether you're scaling up for in vivo studies, kit production, or IND-enabling research, AtaGenix is equipped to be your long-term manufacturing partner.

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