Reducing Immunogenicity While Preserving Affinity
Overview
Therapeutic and diagnostic antibody development increasingly relies on engineering strategies that ensure efficacy, safety, and developability. Among these, antibody humanization is a critical step for minimizing immunogenicity of non-human antibodies while maintaining antigen-binding performance.
At AtaGenix, we provide customized antibody humanization and sequence optimization services using a structure-guided, in silico-assisted approach. Our platform combines CDR grafting, back mutation prediction, and post-translational modification (PTM) risk analysis, delivering optimized antibody variants with reduced immunogenicity and preserved binding affinity.
Why Antibody Humanization?
Antibodies derived from mouse, rabbit, or camelid systems often trigger immune responses when administered to humans. Humanization addresses this by replacing the non-human framework regions with human germline sequences while retaining the antigen-binding CDRs.
Benefits of humanization include:
Our Strategy
AtaGenix employs a comprehensive antibody humanization strategy, integrating:
This multi-layered workflow ensures minimal affinity loss and supports a wide range of antibody formats, including IgG, scFv, Fab, and VHH.
Project Workflow
| Step | Description | Timeline | Deliverables |
|---|---|---|---|
| 1. Sequence Analysis | Human germline selection, CDR grafting, structure modeling, PTM screening | 2–3 days | Optimized humanized sequences |
| 2. Gene Synthesis & Expression | Codon optimization, vector construction, transient expression | ~2 weeks | Expressed antibody variants |
| 3. Affinity Validation | Binding confirmation using BLI or SPR | ~1 week | KD report, binding curves |
| 4. Optional Add-ons | Stability testing, functional assay (FACS, ELISA) | On request | Stability, functional reports |
Total turnaround: typically 3–4 weeks
Key Features
Deliverables
Why Partner with AtaGenix?
As a CRO rooted in antibody engineering, AtaGenix offers more than just design tools. Our platform includes full downstream validation capacity and technical consultation with PhD-level scientists experienced in:
With over 14 years of continuous work in antibody development, our humanization service is grounded in real-world experience and optimized for project efficiency.
Supported Formats
Get Started Today
Let’s accelerate your antibody development journey! Partner with AtaGenix to transform your non-human antibodies into safe, effective, and humanized therapeutics or diagnostics. Contact us now to discuss your project needs and receive a tailored proposal within 24 hours.
