AtaGenix Laboratories
AtaGenix Laboratories
AtaGenix builds custom immune phage display libraries from immunized donors when your project requires higher target-specific affinity than naïve libraries can deliver. Immune libraries are constructed from B cells that have undergone in vivo antigen-driven affinity maturation — meaning even smaller libraries (10⁶–10⁸) yield high-affinity binders for your specific target.
7–10
Weeks to Library
5+
Species Supported
scFv/Fab/VHH
Library Formats
High
Target-Specific Affinity
We construct immune libraries from rabbit, mouse, sheep, alpaca, llama, and other species — in scFv, Fab, or VHH format. Immune libraries are the preferred route when working with small or modified antigens (haptens, PTMs), when sub-nanomolar affinity is required from the first screen, or when rabbit monoclonal antibodies are needed (since rabbit hybridoma is patent-restricted).
| Factor | Immune Library | Naïve Library |
| Immunization | Required (4–6 weeks) | Not needed |
| Target-Specific Affinity | High (in vivo matured) | Moderate (may need maturation) |
| Library Size Needed | 10⁶–10⁸ (smaller is sufficient) | 10⁹+ (diversity compensates) |
| Small / Modified Antigens | Excellent (immune response focused) | Challenging (low hit rate) |
| Species Flexibility | Any immunizable species (no cell fusion needed) | Limited to pre-built libraries |
| Timeline | 7–10 weeks (library build) | 2–3 weeks (ready to pan) |
In Vivo Matured = Higher Affinity
B cells from immunized donors have already undergone somatic hypermutation and clonal selection in vivo. The resulting library is pre-enriched for high-affinity, target-specific clones — reducing the number of panning rounds needed and increasing hit quality.
Any Species, No Cell Fusion
Build immune libraries from rabbit, mouse, sheep, alpaca, llama, or other species. Unlike hybridoma, phage library construction requires no myeloma partner cell line — making it the only practical route to rabbit monoclonal antibodies via immunization.
Difficult Antigens Welcome
Small molecules, haptens, PTM-bearing peptides, and poorly immunogenic targets benefit most from immune libraries. The focused immune response generates clones that naïve libraries — with their broader but shallower diversity — often miss.
AtaGenix constructs custom immune phage display libraries from immunized donors of any species (rabbit, mouse, sheep, alpaca, llama, etc.) in scFv, Fab, or VHH format. The standard workflow covers immunization (4–6 weeks), PBMC/spleen isolation, total RNA extraction, cDNA synthesis, VH/VL PCR amplification, phagemid assembly, and library transformation. Total library construction time: 7–10 weeks. The resulting library is then available for biopanning against your target, followed by monoclonal ELISA screening, sequencing, and optional recombinant expression.
A 4-stage pipeline from immunization to ready-to-pan library. Total construction time: 7–10 weeks. Biopanning and screening follow as a separate phase.
01
Immunization
4–6 weeks
Antigen preparation
Animal immunization
Titer monitoring by ELISA
02
B Cell Isolation
~1 week
Spleen + bone marrow harvest
PBMC isolation
Total RNA extraction
cDNA synthesis
03
PCR & Assembly
~1 week
VH and VL amplification
scFv / Fab / VHH assembly
Phagemid cloning
04
Library QC
1–2 weeks
Transformation & titer
Insert rate verification
Diversity analysis
Library ready to pan
Service Scope
| ✓ Antigen preparation (or client-provided) | ✓ Immunization + titer monitoring |
| ✓ Species: rabbit, mouse, sheep, alpaca, llama, etc. | ✓ Formats: scFv, Fab, VHH |
| ✓ PBMC / spleen isolation + RNA extraction | ✓ VH/VL PCR + phagemid assembly |
| ✓ Library QC: titer, insert rate, diversity | ✓ Biopanning available as add-on service |
| ✓ Recombinant expression + functional validation | ✓ Full sequence data + diversity report |
Need faster results without immunization? Screen our 9 pre-built naïve libraries (human, VHH, rabbit, dog, cat) for hits in 2–3 weeks. Or combine both: naïve library for quick leads + immune library for high-affinity follow-up.
Why build an immune library instead of using a naïve library?
Immune libraries contain B cell repertoires that have undergone in vivo antigen-driven affinity maturation. This means even smaller libraries (10⁶–10⁸) yield high-affinity, target-specific binders — particularly important for small molecules, haptens, PTMs, and poorly immunogenic targets where naïve libraries often produce only moderate-affinity hits.
Which species can you build immune libraries from?
We routinely build immune libraries from rabbit, mouse, sheep, alpaca, and llama. Other species are available on request. Importantly, phage library construction does not require a species-specific myeloma cell line — making it the only practical route to immunized rabbit monoclonal antibodies, since rabbit hybridoma technology is patent-restricted.
How long does immune library construction take?
7–10 weeks total: immunization (4–6 weeks), PBMC/spleen isolation + RNA extraction + cDNA synthesis (~1 week), VH/VL PCR amplification (~1 week), and phagemid assembly + library QC (1–2 weeks). Biopanning against your target follows as a separate phase (2–4 additional weeks).
What QC data is provided for the constructed library?
Every library delivery includes: library titer (pfu/mL), insert rate verification (% clones with correct insert), sequence diversity analysis (CDR3 length distribution, germline usage), and a comprehensive construction report. The library is delivered ready for biopanning with full documentation.
Library diversity and hit rates are target- and species-dependent. Immune libraries are recommended when naïve library hits lack sufficient affinity, or when working with challenging antigens. Cross-link URLs should be confirmed with actual page IDs. Quote-based pricing.
Contact Us
+86-27-87001869
info@atagenix.com
Building C, R & D Building, No. 666, Shendun 4th Road, Donghu New Technology Development Zone, Wuhan
