AtaGenix Laboratories

Home - Antibody Discovery

Immune Library Construction & Panning

AtaGenix offers high-diversity antibody libraries for phage display, providing a rapid and efficient solution for antibody discovery. Compared to immune libraries, which require prior immunization and longer development time, naïve libraries enable faster screening while maintaining high specificity and affinity. However, immune libraries offer advantages for high-affinity binders, particularly for small or modified antigens, and generate more target-specific antibodies. Additionally, immune libraries allow the use of various species without the need for cell fusion, making them ideal for rabbit monoclonal antibody development. AtaGenix provides tailored solutions for both naïve and immune libraries, ensuring optimized antibody selection based on project requirements.

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AtaGenix is an expert in antibody production, offering diverse antibody libraries from various species, including Rabbit, Mouse, Sheep, Alpaca, and Llama (for VHH libraries), tailored to your research needs.

Immunization

  • 4-6 weeks
 

Isolation of PBMC

  • Spleen + Bone marrow
  • Total RNA extraction
  • cDNA synthesis
  • 1 week
 

PCR Amplification of Antibodies

  • VH and VL amplification
  • 1 week
 

cDNA VH/VL Assembling & Library Construction

  • Phagemid synthesis and cloning
  • Transformation for production
  • 1-2 weeks
 

Total

  • 7-10 weeks

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