E. Coli

AtaGenix provides high-quality protein production services in E. coli, ensuring cost-effective solutions, optimized gene synthesis, and efficient codon adaptation. Our customized expression packages offer up to 32 expression condition tests, scalable production from milligram to gram levels, and strict quality control (QC) analysis. With the support of PhD-level experts, AtaGenix delivers reliable, high-yield protein expression tailored to your research and biopharmaceutical needs.

AtaGenix offers a streamlined E. coli protein expression workflow, ensuring high-yield production, optimized gene synthesis, and scalable purification. Our process includes:

Codon-optimized gene synthesis for efficient protein expression.
Plasmid transformation into bacterial strains.
Evaluation of 32 expression conditions to determine optimal parameters.
Scalable expression & purification from milligrams to grams.
Comprehensive QC analysis (SDS-PAGE, Western Blot, Bradford).

With cutting-edge expression optimization and PhD-level support, AtaGenix ensures fast, cost-effective, and reliable protein production.

Expression Vector Construction

Gene synthesis including codon optimization for E. coli production.
Gene subcloning in a high-producing expression vector.

E. coli Strain Transformation

Transformation of the plasmid into various bacterial strains.

Test of 32 Protein Expression Conditions

Evaluation of 32 protein expression conditions (temperature, induction time, fractions).
Determination of the best protein production conditions.
Protein purification tests.

Protein Expression Scale-up & Purification

1L protein expression and purification with optimized conditions.

QC Analysis

SDS-PAGE analysis and Western Blot.
Quantitative analysis by the Bradford method.

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