Optimizing Antibody-Antigen Binding Through Rational Engineering
Overview
Affinity maturation enhances therapeutic and diagnostic antibodies by improving binding strength to target antigens, boosting efficacy, reducing doses, and aiding downstream development.
At AtaGenix, we provide end-to-end services with advanced display systems, mutagenesis, and real-time binding analysis to transform leads into optimized candidates.
Why Affinity Maturation?
Enhance KD values by 10–100 fold
Fine-tune epitope recognition and selectivity
Improve sensitivity in ELISA, IHC, or flow cytometry
Support species cross-reactivity or mutation-tolerant binding
Rescue weak or borderline clones
Our Workflow
Step-by-Step Process
| Milestone | Description | Key Output |
|---|---|---|
| Sequence Engineering | Site-directed mutagenesis or error-prone PCR on CDRs | CDR-scFv/Fab/VHH libraries |
| Library Screening | Phage or mammalian display, ELISA/FACS selection | Candidate pools |
| Hit Validation | Secondary screening via BLI/SPR | Kinetic data |
| Combination Design | Hotspot mutation combination & synthesis | Optimized panel |
| Expression & QC | HEK293/CHO expression and quality profiling | Purified high-affinity clones |
Technology Highlights
Full CDR saturation mutagenesis (CDR1–3)
Phage and mammalian display systems
High-throughput ELISA, SPR, BLI, and FACS platforms
6–8 week typical turnaround
Deliverables
Affinity-matured antibody sequences
BLI or SPR kinetic data
Expression and yield reports
Thermostability and solubility data (optional)
Final technical report and recommendations
Supported Formats:
IgG, scFv, Fab, VHH, bispecifics, Fc-fusions
Why AtaGenix?
14+ years of experience in antibody engineering
In-house display and high-throughput screening
GMP-compliant facilities with ISO workflows
Direct consultation with scientific leads
Flexible project design and milestone-based billing
“Affinity maturation is about understanding epitope context, developability, and application fit.”
— Dr. Olivier Leger, Antibody Engineering Advisor, AtaGenix
Use Cases
Affinity improvement of immunized antibody leads (rabbit, mouse, human)
Rescuing weak scFv/VHH clones for CAR-T, bispecifics, or ADC development
Improving diagnostic antibodies with low affinity
Epitope tuning for cross-reactive or mutated targets
Contact Us
Enhance your antibody’s affinity with a trusted CRO partner.
? info@atagenix.com
Submit your project via our contact form for a proposal.
