AtaGenix Laboratories

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Yeast

AtaGenix combines its extensive expertise in protein production with a comprehensive toolbox to optimize your yeast protein expression.

Why Choose AtaGenix for Your Yeast Protein Expression?

Choose between several hosts

Choose between P. pastoris and S. cerevisiae for your protein expression.

Access a complete yeast expression toolbox

We propose different strains of P. pastoris or S. cerevisiae to optimize your protein production.

Unrivalled protein production expertise

With 1500+ protein expressions, you can trust our expertise.

Integrated solution from gene to protein

From optimized gene to protein, we are your protein expression partner!

PhD account managers

At AtaGenix, a PhD account manager assists you!

Cost-effective expression system

Yeasts are known for their fast growth rate and high yield protein expression.

Our Yeast Expression Process

Expression vector construction (optional)
- Gene design including codon optimization
- Gene synthesis
- Subcloning in an expression vector

Small-scale protein expression
- Transformation and selection of yeast clones
- Determination of top expressing clones
- Expression optimization
- SDS-page + WB if necessary
- Purification tests

Protein expression scale up and purification
- Protein expression and purification with optimized conditions previously determined.

Our Yeast Protein Expression Workflow

Step Content Timeline Deliverables
Protein pilot - Transformation of yeast cells
- Selection of the best expressing clones
- Protein expression
- Optimization
- Purification tests
4 to 5 weeks - Test protein sample
Transient protein expression and purification - Large scale protein expression
- Purification
- QC analysis: SDS-PAGE, concentration
2 weeks - Purified protein
- Detailed report

 

Optional needs including codon optimization for protein expression and subcloning in an expression vector

- Protein expression chromatography
- DNA, HPLC, endotoxins
- Analytics: WB, analytical SEC

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