AtaGenix Laboratories
AtaGenix combines its extensive expertise in protein production with a comprehensive toolbox to optimize your yeast protein expression.
Choose between several hosts
Choose between P. pastoris and S. cerevisiae for your protein expression.
Access a complete yeast expression toolbox
We propose different strains of P. pastoris or S. cerevisiae to optimize your protein production.
Unrivalled protein production expertise
With 1500+ protein expressions, you can trust our expertise.
Integrated solution from gene to protein
From optimized gene to protein, we are your protein expression partner!
PhD account managers
At AtaGenix, a PhD account manager assists you!
Cost-effective expression system
Yeasts are known for their fast growth rate and high yield protein expression.
Expression vector construction (optional)
- Gene design including codon optimization
- Gene synthesis
- Subcloning in an expression vector
Small-scale protein expression
- Transformation and selection of yeast clones
- Determination of top expressing clones
- Expression optimization
- SDS-page + WB if necessary
- Purification tests
Protein expression scale up and purification
- Protein expression and purification with optimized conditions previously determined.
Step | Content | Timeline | Deliverables |
Protein pilot | - Transformation of yeast cells - Selection of the best expressing clones - Protein expression - Optimization - Purification tests |
4 to 5 weeks | - Test protein sample |
Transient protein expression and purification | - Large scale protein expression - Purification - QC analysis: SDS-PAGE, concentration |
2 weeks | - Purified protein - Detailed report |
Optional needs including codon optimization for protein expression and subcloning in an expression vector
- Protein expression chromatography
- DNA, HPLC, endotoxins
- Analytics: WB, analytical SEC