AtaGenix Laboratories

Home - Mammalian

CHO Transient

AtaGenix offers high-performance mammalian protein expression solutions, optimizing yield and efficiency with cutting-edge technology. Our XtenCHO™ system ensures superior protein expression, while our CHO and HEK platforms provide versatile options tailored to different protocols and tools. With large-scale production capabilities, our cell cultures reach 20L per batch, delivering multi-gram quantities of protein in just 4 weeks. We support the entire process, from transient to stable expression, ensuring customized solutions that meet your requirements. Backed by 1500+ successful protein projects and a 90%+ success rate, our expertise is unmatched. AtaGenix provides a fully integrated service, managing your project from gene synthesis to protein expression for seamless bioproduction.

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AtaGenix delivers high-yield protein expression with a 90% success rate and over 1500 proteins expressed, showcasing the quality of our proprietary protein expression systems. Our expert team and dedicated production platforms ensure superior mammalian and baculovirus-based expression. Access our comprehensive data report, featuring detailed test results, purification insights, and process analytics for HEK-based expression projects.

 

Our Protein Expression in Mammalian Cells Workflow

 
Step Content Timeline Deliverables
Gene Synthesis ✔ Gene synthesis
✔ Codon optimization
✔ Subcloning into expression vector
4 to 5 weeks ✔ Protein test sample
Pilot Study ✔ Plasmid amplification
✔ Transfection
✔ Small-scale protein expression
4 to 5 weeks ✔ Purified protein
✔ Detailed report
Transient Protein Expression & Purification ✔ Large-scale protein expression (1L)
✔ Affinity purification
✔ QC analysis: SDS-PAGE, concentration
3 to 4 weeks ✔ Purified protein
✔ Detailed report
Stable Cell Pool Generation ✔ Stable transfection
✔ Pool selection
Pool selection ✔ Stable pool vials
✔ Detailed report
Stable Cell Line Generation ✔ Best clone selection
✔ Clone characterization
✔ Stability study
✔ RCB preparation
✔ Process optimization
20 weeks ✔ RCB tested for mycoplasma
✔ COA
✔ Detailed report with optimized protocol

Options

  • Large-scale transient protein expression: 5L, 10L, 20L
  • Size exclusion chromatography
  • Polishing (DNA, HCP, endotoxins)
  • Endotoxin removal
  • Analytics: WB, analytical SEC

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