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Stable Cell Line Development for Antibodies and Viral Proteins | AtaGenix Case Studies

Release time: 2024-11-05   View volume: 538

Case Study 1: Antibody Stable Cell Line Development

Construction of CHO stable cell lines producing recombinant antibodies, with maximum expression levels exceeding 3 g/L.

Fig 1. Antibody Monoclonal Yield Analysis

Fig 1. Antibody Monoclonal Yield Analysis. Different clones show yield differences, with top performers exceeding 3 g/L, demonstrating high productivity.

Fig 2. Stability Analysis of Antibody Clones

Fig 2. Stability Analysis of Antibody Clones. Expression levels remain consistent across passages, ensuring long-term stability of selected clones.

Fig 3. Mycoplasma Testing of Cell Banks (PCR Method)

Fig 3. Mycoplasma Testing of Cell Banks (PCR Method). No contamination detected, confirming biosafety and reliability of the CHO cell banks.

MW: DL2000 Marker. +: Positive control (290 bp). -: Negative control.

Fig 4. Antibody Purity Analysis of Top1 Cell Line

Fig 4. Antibody Purity Analysis of Top1 Cell Line. High purity confirmed by SDS-PAGE, showing minimal impurities and consistent antibody quality.

Top1 Cell Line Antibody Affinity Detection
Sample ka (1/Ms) kd (1/s) KD (M)
12-10 1.29E+05 9.52E-06 7.36E-11

Fig 5. Top1 Cell Line Antibody Affinity Detection. Binding kinetics confirm strong affinity, validating clone selection for therapeutic development.

Case Study 2: Stable Cell Line for Viral Protein Expression

Expression results of stable cell line producing Varicella-Zoster Virus gE protein.

Fig 1. QC SDS-PAGE Analysis of Different Clones

Fig 1. QC SDS-PAGE Analysis of Different Clones. Distinct bands confirm expression of gE protein, supporting downstream vaccine-related studies.

Lane MW: Protein Marker

Need reliable cell line development for antibody or viral protein projects? AtaGenix provides custom solutions from gene design to stable cell bank validation.

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