AtaGenix’s Custom Phospho-USP8 Antibody Advances MDA5 Homeostasis Research, Unveiling a Novel Target for Autoimmune Therapy

Client Requirements
The research team required a phospho-specific antibody to detect USP8 Ser718 phosphorylation, validated for Western blotting, to study AKT–USP8–MDA5 regulation in viral infection models.

Technical Challenges
1. Antibody specificity: Distinguish phosphorylated USP8 Ser718 from non-phosphorylated forms.
2. Application compatibility: Perform reliably in Western blotting and Co-IP assays.
3. Batch consistency: Ensure reproducibility across multiple research stages.

Custom Antibody Solution

1. Antibody Development:
– Immunization with phosphorylated USP8 Ser718 peptide (Cys-REPSKLKRSYS(p)SPDI) in rabbits.
– Affinity purification ensured specificity for the phospho-epitope.

2. Validation and Support:
– Validated in HEK293T and THP1 cells for phosphorylation detection.
– Applied in Co-IP and Western blot assays, with full protocol and support provided.

The antibody enabled researchers to confirm how AKT-mediated USP8 phosphorylation regulates MDA5 stabilization, providing critical insights into autoimmune disease. This solution supported their Advanced Science publication.