SARS-CoV-2 Spike Protein Expression & Purification | AtaGenix High-Yield Mammalian System

Using a mammalian expression workflow, the SARS-CoV-2 spike (S) protein was produced at high yield and purified to high purity. Elution fractions showed strong target bands with minimal background, indicating an efficient capture and wash sequence suitable for downstream assay development (e.g., ELISA and rapid diagnostic reagents).

Figure 1. Purification profile of SARS-CoV-2 S protein (mammalian expression). Clear, intense bands appear in E1–E8 with low impurities; later fractions taper as expected. This pattern supports a high-purity, high-yield recovery suitable for diagnostic use.

Lane descriptions.

• MW: Protein marker
• IN: Input (post-expression lysate/supernatant)
• FT: Flow-through (unbound fraction)
• W1, W2: Wash fractions (removal of non-specific proteins)
• E1–E12: Elution fractions (target enrichment; strongest bands in E1–E8)

Interpretation. Strong early-elution bands with clean backgrounds indicate effective binding and wash conditions; diminishing signal in late elutions reflects expected gradient completion. Overall, the dataset supports a robust, scalable process for producing assay-grade S protein.