Precision Solutions

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  Custom Phospho-USP8 (Ser718) Antibody Reveals AKT–MDA5 Axis in Autoimmune Disease

  AtaGenix developed a custom phospho-specific antibody against human USP8 Ser718, enabling an Advanced Science (IF 14.3) study that identified USP8 as the core deubiquitinase governing MDA5 homeostasis. The AKT–USP8–MDA5 axis drives type I interferon signaling; inhibiting USP8 or AKT suppresses MDA5-induced autoimmunity in AGS mice and anti-MDA5-positive DM/SLE patient cells. DOI: 10.1002/advs.202503865.

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  Simvastatin Reverses Ferroptosis Resistance in AKT-Hyperactivated ICC via pPCK1–pLDHA–SPRINGlac Axis

  A Cancer Communications study mapped the pAKT–pPCK1–pLDHA–SPRINGlac axis as a driver of ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma. Simvastatin disrupted mevalonate flux and restored therapy sensitivity in preclinical models. pAKT–pPCK1 emerged as a biomarker for patient stratification. AtaGenix provided custom phospho-specific antibodies (anti-pPCK1 Ser90, anti-pLDHA Thr248) validated for WB/IP/IHC. DOI: 10.1002/cac2.70036.

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  Custom Cit202 Antibody Reveals PAD1–AKT2 Citrullination Axis in Ovarian Cancer Stemness

  An Advanced Science study (IF 14.3) identified the PAD1–AKT2(R202)–CEBPβ axis as a driver of ovarian cancer stem-like cell stemness and cisplatin resistance. PAD1 citrullinates AKT2 at Arg202, maintaining phosphorylation site exposure and activating PI3K-AKT–CEBPβ signaling. PAD1 inhibition re-sensitizes cisplatin-resistant cells. AtaGenix provided a custom site-specific Cit202 antibody validated for co-IP and IHC across 66 clinical samples. DOI: 10.1002/advs.202501014.

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  Custom Anti-cGAS Antibody Enables E2-CBASS Anti-Phage Immunity Discovery

  A Nature Microbiology study revealed that the E2 enzyme in E2-CBASS regulates cGAS via ubiquitination mimicry, promoting poly-cGAS formation (4.2× increase) and cGAMP synthesis (2.3× boost) to confer anti-phage immunity. Cryo-EM resolved the cGAS-E2 covalent complex. AtaGenix provided a custom rabbit anti-cGAS polyclonal antibody that distinguished cGAS monomers (46 kDa), complexes (64 kDa), and poly-cGAS (>100 kDa) in Western blot. DOI: 10.1038/s41564-024-01684-z.