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XtenCHO™ High-Density Transient Transfection Expression System

Release time: 2024-11-13   View volume: 2202

Service Overview — XtenCHO™ is AtaGenix’s proprietary high-density transient transfection CHO cell expression system. Engineered for high-throughput protein and antibody production, XtenCHO™ achieves typical yields of 200–400 mg/L, with select antibodies reaching up to 1 g/L — combining simplified workflows, extended cell viability (10–14 days vs. conventional 6–7 days), and significant cost advantages over standard cationic lipid-based systems.

Service Advantages

Engineered CHO Cell Line

XtenCHO™ cells are genetically optimized with specialized vectors that increase intracellular plasmid copy number and extend retention time, enabling high-level, stable gene expression.

Extended Cell Viability

High-density transfection technology and optimized culture protocols extend post-transfection cell survival from 6–7 days to 10–14 days, significantly boosting protein yield per batch.

Cost-Optimized Production

Proprietary transfection reagents, culture media, and feed supplements deliver substantial cost savings vs. standard commercial systems — scalable from research to industrial production.

Service Workflow

Service Step Client Provides Details Timeline Deliverables
Gene Synthesis Gene sequence, vector preference Codon optimization & gene synthesis 2–3 weeks Synthesis report
Expression & Purification Test Small-scale expression screening & purification optimization 2 weeks Expression & purification report
1 L Scale-Up 1 L expression & multi-step purification 2 weeks Protein samples + QC report
Bioreactor Scale-Up Shaking flask or bioreactor production 2–4 weeks Protein samples + full report

Key Metrics

200–400

mg/L Typical Yield

1 g/L

Peak Antibody Yield

10–14

Days Cell Viability

6–9

Weeks End-to-End

Need high-yield CHO transient expression for antibody screening, protein production, or scale-up? Share your sequence and we’ll propose a XtenCHO™ strategy within 24 hours.

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