How was the anti-human CD86 rabbit monoclonal antibody generated?

Rabbits with serum titers >128K were selected. PBMCs were isolated and flow-sorted to obtain human CD86 antigen-specific single B-cell plates, followed by clone screening.

How was functionality validated?

Immunofluorescence (IF) demonstrated high sensitivity and precise antigen localization; endogenous Western blot (WB) showed a clear primary band without nonspecific binding in Daudi and Raji lysates.

What applications are supported?

The antibody supports IF and endogenous WB, and can be further qualified for flow cytometry, ELISA, and IP upon request.

Xten™ Mab Single B Rabbit Monoclonal Antibody Development Case Study

Q: Which expression system was used?

Positive clones were expressed with the AtaGenix XtenCHO high-density transient expression system prior to purification and functional validation.

Release time: 2024-11-04 View volume: 549

Project Summary

Utilizing rabbit single B-cell antibody technology, an Anti-human CD86 rabbit monoclonal antibody was developed. Rabbits with serum titers greater than 128K were selected, and PBMCs were isolated for flow sorting to obtain human CD86 antigen-specific B-cell plates.

Fig 1. Flow Sorting Results of Rabbit Single B-Cells

Fig 1. Flow Sorting Results of Rabbit Single B-Cells

The XtenCHO high-density transient expression system by AtaGenix was used for recombinant expression of positive clones. Purified antibodies were then validated for functionality.

Fig 2. Analysis of Recombinant Expression of Rabbit Monoclonal Antibodies

Fig 2. Analysis of Recombinant Expression of Rabbit Monoclonal Antibodies

Purified antibodies were further validated via IF and endogenous WB assays. IF results showed high sensitivity and precise antigen localization, while WB analysis revealed a clear primary band with no nonspecific binding.

Fig 3. IF Analysis of Anti-human CD86 Rabbit Monoclonal Antibody