AtaGenix Laboratories
AtaGenix Laboratories
Release time: 2024-11-04 View volume: 863
Project Snapshot — Using rabbit single B-cell technology, AtaGenix developed an anti-human CD86 rabbit monoclonal antibody. High-titer rabbits (serum titer >128K) were flow-sorted for antigen-specific B-cells, positive clones expressed via the XtenCHO™ transient system, and purified antibodies validated by immunofluorescence (IF) and endogenous Western blot (WB) with high specificity.
Rabbits immunized with human CD86 antigen were screened for serum titers greater than 128K. PBMCs were isolated and flow-sorted to obtain antigen-specific single B-cell plates for downstream clone identification.
Fig 1. Flow sorting results of rabbit single B-cells. Antigen-specific B-cells were gated and sorted from high-titer rabbit PBMCs.
Positive clones were recombinantly expressed using the AtaGenix XtenCHO™ high-density transient expression system. Purified antibodies were then advanced to functional validation.
Fig 2. SDS-PAGE / SEC analysis of recombinant rabbit monoclonal antibody expression and purification.
Purified antibodies were validated via immunofluorescence and endogenous Western blot. IF results demonstrated high sensitivity and precise antigen localization on cell membranes, while WB analysis showed a clear primary band with no nonspecific binding.
Fig 3. Immunofluorescence analysis of anti-human CD86 rabbit mAb. RAJI cells, primary antibody 1:50, secondary antibody 1:50, exposure time 30 ms.
Fig 4. Endogenous Western blot analysis of anti-human CD86 rabbit mAb. Lane 1: Daudi cell lysate; Lane 2: Raji cell lysate. Clear primary band with no nonspecific binding.
>128K
Serum Titer
IF + WB
Validated Applications
Rabbit
Single B-Cell Platform
XtenCHO
Expression System
Results may vary depending on target, immunization strategy, and project scope. All proprietary client information is subject to NDA.
Need rabbit monoclonal antibodies from single B-cell discovery to XtenCHO™ expression and validation? AtaGenix provides end-to-end development with assay-ready QC.
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+86-27-87001869
info@atagenix.com
Building C, R & D Building, No. 666, Shendun 4th Road, Donghu New Technology Development Zone, Wuhan
