Prokaryotic Protein Expression (E. coli) — 3H Platform with >95% Success Rate

Service Overview — AtaGenix’s 3H E. coli expression platform (High Throughput, High Efficiency, High Quality) achieves >95% expression success rate across tens of thousands of delivered projects. Featuring optimized vectors (pET, pGEX series), specialized host strains (T7E, C41, Arctic, proprietary AtaGenix strains), and a complete expression–purification–endotoxin control pipeline, the system delivers purity ≥85% and endotoxin <0.1 EU/mL from milligram to gram scale — ISO 9001 & ISO 13485 certified.

Background: This study cloned the key gene SDR3.1 (encoding the MODD protein) and elucidated the molecular mechanisms of pre-harvest sprouting (PHS) in rice, providing tools for genetic improvement of PHS resistance.

AtaGenix’s Role: Produced recombinant SDR3.1 protein via E. coli expression, then used it as an immunogen to develop a rabbit monoclonal anti-SDR3.1 antibody. The antibody performed at 1:10,000 dilution in WB, accurately detecting SDR3.1 expression and phosphorylation across rice varieties (ZH11, MJLXL) and transgenic plants — directly validating that SDR3.1 negatively regulates seed dormancy by inhibiting ABI5 transcriptional activity.

Published in Nature Communications (IF: 14.7, Q1) — DOI: 10.1038/s41467-024-45402-z

Fig. 1. Fine mapping of SDR3.1. Anti-SDR3.1 antibody produced from E. coli-expressed recombinant protein. Adapted from Nat Commun 2024.

Background: This research revealed how the transcription factor FTZ-F1 mediates non-fitness-cost resistance to Bt Cry1Ac toxin in Plutella xylostella, identifying new targets for eco-friendly pest control.

AtaGenix’s Role: Expressed recombinant FTZ-F1 protein in E. coli, then produced a rabbit polyclonal anti-FTZ-F1 antibody via Protein A/G purification. The antibody distinguished phosphorylated vs. non-phosphorylated FTZ-F1 by Phos-tag SDS-PAGE and performed at 1:10,000 in WB — confirming that MAPK-mediated FTZ-F1 phosphorylation drives differential Bt receptor gene expression.

Published in Nature Communications (IF: 14.7) — DOI: 10.1038/s41467-022-33706-x

Fig. 2. FTZ-F1 protein expression and phosphorylation in larval midgut of five P. xylostella strains. Anti-FTZ-F1 antibody from E. coli-expressed antigen. Adapted from Nat Commun 2022.

Background: This study established a hypermutation system in Synechococcus elongatus PCC 7942, screened mutants tolerant to high-light and high-temperature (HLHT) stress, and dissected photosynthetic adaptation mechanisms under extreme conditions.

AtaGenix’s Role: Expressed and purified key proteins — RecA, UmuDC (DNA replication fidelity), and shikimate kinase (downstream validation) — via the E. coli system. These high-activity recombinant proteins supported hypermutation system construction and confirmed that shikimate kinase overexpression enhances HLHT tolerance by remodeling the photosynthetic chain and metabolic network.

Published in Nature Communications (IF: 14.7) — DOI: 10.1038/s41467-023-36964-5

Fig. 3. WB (top) and SDS-PAGE (bottom) of shikimate kinase in HS199 strain. E. coli-expressed by AtaGenix. Adapted from Nat Commun 2023.

From recombinant protein production and antibody immunogen preparation to functional validation — AtaGenix’s 3H E. coli platform delivers reliable support at every stage.

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