Protein A and Protein B Expression in Bacillus subtilis | High-Purity Recombinant Protein Solutions by AtaGenix

Case Study 1: Protein A Expression and Secretion in Bacillus subtilis

After synthesizing the Protein A gene, it was subcloned into the expression vector pHT43 and transformed into Bacillus subtilis WB800N. Successful protein secretion was achieved (~85 kDa; N-terminal 6*His) with a yield of 20 mg/L and purity above 90%.

Protein A QC (Reduced SDS-PAGE)
Lane M: Protein marker; Lane Ø: Blank control; Lane 1-6: Supernatant and lysate from different clones.

Case Study 2: Protein B Intracellular Expression in Bacillus subtilis

After synthesizing the Protein B gene, it was subcloned into the expression vector pHT254 and transformed into Bacillus subtilis WB800N. Successful intracellular expression was achieved (~42 kDa; C-terminal 6*His) with a yield of 1 mg/L and purity above 95%.

Protein B QC (Reduced SDS-PAGE)
Lane M: Protein marker; Lane Ø: Blank control; Lane +: Positive control; Lane 2-4: Lysate supernatant and precipitate from different clones.