RTiterFast® Adjuvant – High-Performance Aqueous Adjuvant for Rabbits

ATO00071

RTiterFast® Adjuvant

Rapid Aqueous Adjuvant for Rabbits | pH-Neutral, Non-Toxic | 1,000+ Antibodies Developed

Product Description

RTiterFast® Adjuvant is a pH-neutral aqueous adjuvant with a bluish transparent appearance. Its primary component is a high-molecular-weight polymer whose hydrophobic ends bind to protein antigens without altering their structure. Once injected, the adjuvant slowly releases the bound antigen, gradually stimulating the immune system.

All auxiliary materials meet human-use pharmaceutical standards — non-toxic, non-immunogenic, and highly biocompatible. Significantly reduces adverse reactions (inflammation, granulomas, injection site necrosis) compared to Freund’s adjuvant. Contains no protein components, preventing antibody production against the adjuvant. RTiterFast® is primarily designed for use in rabbits.

Tested in 1,972 New Zealand White rabbits with various antigen types (recombinant proteins, peptides, small molecules, inactivated whole viruses, VLPs). Over 1,000 high-quality antibodies have been developed, validated in WB, IHC, IP, IF, Sandwich ELISA, indirect ELISA, and Neutralization assays.

Experimental Validation

RTiterFast Aqueous Adjuvant for Rabbits - Serum Titer Distribution

Fig 1. Serum titer distribution of 1,972 NZW rabbits immunized 3× with RTiterFast®. ~83% recombinant proteins, ~16% peptides, ~1% small molecules/viruses/VLPs. Cutoff OD = 0.25.

Key Advantages

Proven at Scale 1,972 rabbits tested; 1,000+ high-quality antibodies developed across multiple applications.

Superior Safety No granulomas, no injection site necrosis. Gentle aqueous formulation with excellent absorption.

Cost-Effective Reduces antigen consumption and shortens immunization cycles vs. Freund’s adjuvant.

No Adjuvant Antibodies Protein-free formulation prevents interference from anti-adjuvant antibody production.

Ordering Information

Cat. No.	Product Name	Size	Price (USD)
ATO00071	RTiterFast Adjuvant (Rapid Adjuvant for Rabbits)	2 mL	$160
ATO00071	RTiterFast Adjuvant (Rapid Adjuvant for Rabbits)	5 mL	$360

Usage Guidelines

Immunization Protocol: Three immunizations recommended (Day 0, Day 7, Day 14). Collect serum on Day 21 for titer evaluation. If suboptimal, administer booster on Day 21–22.

Antigen Dosage:

Protein antigens / viruses / VLPs: 1st dose 180–220 μg, dilute to 300 μL + 100 μL RTiterFast®. Subsequent doses: 80–120 μg, same prep.
Small molecules / peptides on carriers: 1st dose 400–500 μg, dilute to 300 μL + 100 μL RTiterFast®. Subsequent doses: 250 μg, same prep.

Injection: 400 μL total per rabbit. Intramuscular injection into the calf is recommended for convenience.

Preparation: Dilute antigen with sterile saline or PBS to recommended volume. Mix with adjuvant and inject immediately.

Storage: 4–8°C. Do not freeze. Shelf life: 2 years.

Precautions

Precipitation after mixing is normal. Mix thoroughly before loading syringe; inject immediately.
Optimized for rabbits; may have slightly reduced efficacy in other species.
Handle with care when opening to avoid injury from the aluminum seal.

Need adjuvants for rabbit immunization? Explore our full TiterFast® series.

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For research use only.

Datasheet