Protein A ELISA Kit| High-Sensitivity Detection

ATK00052

Protein A ELISA Kit

Double-Antibody Sandwich ELISA | Sensitivity: 0.05 ng/mL | 96 Wells

Product Description

The Protein A ELISA Kit is designed for quantitative detection of recombinant and alkali-tolerant Protein A in biological samples. Using a double-antibody sandwich ELISA method, this kit ensures high specificity and sensitivity with a detection limit of 0.05 ng/mL. Compatible with humanized monoclonal antibody concentrations up to 2 mg/mL.

Key Specifications

Sensitivity0.05 ng/mL

Wavelength450 nm

PrecisionCV < 10% (> 0.3 ng/mL)

Hook Effect10 μg/mL

Kit Components

Component	Details	Storage
Protein A Standard	Recombinant alkali-tolerant Protein A, 50 μg/mL, 1×0.1 mL	2–8°C
Biotinylated Antibody	Biotinylated anti-Protein A mAb, 1×13 mL	2–8°C
Sample Diluent	Tris buffered saline, 1×30 mL	2–8°C
Denaturing Buffer	Citrate buffer, 1×20 mL	2–8°C
10× Assay Buffer	Phosphate buffer, 1×30 mL	2–8°C
Streptavidin-HRP	Streptavidin-labeled HRP, 1×13 mL	2–8°C
TMB Substrate	TMB, 1×13 mL	2–8°C
Stop Solution	2M H&sub2;SO&sub4;, 1×13 mL	2–8°C
Coated Plate	Anti-Protein A antibody-coated strips, 12×8 wells	2–8°C