cGMP ELISA Kit | High-Sensitivity Detection

ATK00050

cGMP ELISA Kit

Competitive ELISA | Sensitivity: 0.1 pmol/mL | IC50: ~10 pmol/mL

Product Description

cGMP is a cyclic nucleotide second messenger synthesized by guanylyl cyclase and degraded by phosphodiesterase, critical in cardiovascular function and sensory signaling. This kit uses a competitive ELISA method: HRP-conjugated cGMP competes with sample cGMP for binding to anti-cGMP monoclonal antibodies. Absorbance at 450 nm is inversely proportional to cGMP concentration.

Key Specifications

Sensitivity0.1 pmol/mL

IC50~10 pmol/mL

Wavelength450 nm (ref 630 nm)

MethodCompetitive ELISA

Kit Components

Component	Details	Storage
cGMP Standard	10,000 pmol/mL, 1×0.1 mL	−80°C
cGMP-HRP	1000× HRP conjugated cGMP, 1×0.02 mL	−80°C
cGMP Antibody	Anti-cGMP mAb (1000×), 1×0.02 mL	−80°C
10× Assay Buffer	Phosphate buffer with preservative, 1×30 mL	2–8°C
Neutralizing Reagent	Tris buffered saline, 1×8 mL	2–8°C
Sample Diluent	0.1M HCl, 1×30 mL	2–8°C
TMB Substrate	TMB, 1×18 mL	2–8°C
Stop Solution	2M H&sub2;SO&sub4;, 1×8 mL	2–8°C
Coated Plate	Goat anti-Mouse IgG, 96 wells	2–8°C