cAMP ELISA Kit | High-Sensitivity Detection

ATK00049

cAMP ELISA Kit

Competitive ELISA | Sensitivity: 0.15 pmol/mL | IC50: ~12 pmol/mL

Product Description

cAMP is a cyclic nucleotide second messenger produced by adenylyl cyclase, playing a key role in signal transduction pathways across cardiovascular, neurological, and metabolic processes. This kit uses a competitive ELISA: sample cAMP competes with HRP-labeled cAMP for binding to anti-cAMP monoclonal antibodies. Absorbance at 450 nm is inversely proportional to cAMP concentration.

Key Specifications

Sensitivity0.15 pmol/mL

IC50~12 pmol/mL

Wavelength450 nm (ref 630 nm)

MethodCompetitive ELISA

Kit Components

Component	Details	Storage
cAMP Standard	10,000 pmol/mL, 1×0.1 mL	−80°C
cAMP-HRP	1000× HRP conjugated cAMP, 1×0.02 mL	−80°C
cAMP Antibody	Anti-cAMP mAb (1000×), 1×0.02 mL	−80°C
10× Assay Buffer	Phosphate buffer with preservative, 1×30 mL	2–8°C
Neutralizing Reagent	Tris buffered saline, 1×8 mL	2–8°C
Sample Diluent	0.1M HCl, 1×30 mL	2–8°C
TMB Substrate	TMB, 1×18 mL	2–8°C
Stop Solution	2M H&sub2;SO&sub4;, 1×8 mL	2–8°C
Coated Plate	Goat anti-Mouse IgG, 96 wells	2–8°C