AtaGenix Laboratories
AtaGenix Laboratories
Tobacco Etch Virus NIa Protease | Recombinant, E. coli-expressed
Product Description
TEV Protease, derived from Tobacco Etch Virus NIa, is a recombinant cysteine protease with exceptional site specificity. It recognizes the seven-amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln↓Gly (ENLYFQ↓G) and cleaves precisely between Gln and Gly. It efficiently removes affinity tags such as GST or other fusion tags, ensuring precise and effective protein preparation.
Product Advantages
Applications
Product Specifications
| Attribute | Details |
|---|---|
| Product Name | TEV Protease |
| Catalog Number | ATE00001 |
| Host | E. coli |
| Tag | N-terminal GST Tag |
| Cleavage Site | ENLYFQ↓G |
| Molecular Weight | 50 kDa |
| Form | Liquid |
| Buffer | 25 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, 50% glycerol, pH 8.0 |
| Purity | >95% (by SDS-PAGE) |
| Specific Activity | 17 U/μL |
| Unit Definition | Amount required to cleave >85% of 3 μg substrate at 30°C, pH 8.0, in 1 hour |
| Reaction Conditions | 1× TEV Buffer (50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, 1 mM DTT, pH 8.0); 30°C, 1 hour |
| Storage | -20°C; shelf life 12 months |
| Shipping | Blue ice |
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Contact Us
+86-27-6552-3339
info@atagenix.com
Building C, R & D Building, No. 666, Shendun 4th Road, Donghu New Technology Development Zone, Wuhan
