Polyclonal Antibody Production FAQ | Antigen Selection & Purification | AtaGenix

AtaGenix specializes in producing polyclonal antibodies using various antigen types, including peptides, proteins, DNA, small molecules, and cell lysates. Our advanced facilities also support custom antigen production or the use of client-provided antigens.

Maximizing antibody specificity and immune response depends on selecting the right antigen. AtaGenix offers antigen generation for:

• Proteins: The most common choice, ensuring broad epitope exposure.
• Peptides: Custom-synthesized for linear epitopes, ideal for Western Blot and ELISA.
• DNA: Used in special cases where traditional immunization is difficult.
• Small Molecules: Requires conjugation with carrier proteins.

Our standard immunization protocols include:

• 51-day schedule for protein-based polyclonal antibody production.
• 70-day schedule for peptide-based polyclonal antibody production.

Extended immunization schedules are available for higher titers. Lower antigen doses with longer immunization periods generally yield the best results.

After reaching peak antibody titers, AtaGenix purifies polyclonal antibodies using:

• Protein A/G Purification: High-yield purification with minimal non-specific binding.
• Antigen-Specific Affinity Purification: Removes non-specific antibodies for maximum specificity.

We regularly test serum samples from hyperimmunized hosts using ELISA and, upon request, Western Blot for additional validation.