What is a pMHC complex and why refold it?

A pMHC is an HLA heavy chain with β2-microglobulin bound to a peptide. Refolding ensures correct assembly and peptide loading after recombinant expression in E. coli.

Why use streptavidin to make tetramers?

Biotinylated pMHC can be multimerized with streptavidin to form tetramers that increase avidity for TCRs, improving sensitivity in antigen-specific T-cell detection.

Can AtaGenix deliver ready-to-use pMHC tetramers?

Yes. AtaGenix supports expression, refolding, biotinylation, and streptavidin-based tetramer assembly, plus QC (SDS-PAGE, SEC-HPLC, peptide loading verification) to meet assay requirements.

HLA-I Expression and pMHC Tetramer Assembly in E. coli | AtaGenix

Release time: 2024-11-05 View volume: 538

Expression of HLA-I Protein in E. coli – Peptide Complex

Expression and refolding of HLA-I with β2M into stable pMHC complex, tetramerized with streptavidin

Fig. 1: Expression and Refolding of HLA-I Protein

HLA and β2M were expressed and purified in E. coli, then refolded with peptide to form stable pMHC (HLA/β2M/peptide) complexes. Addition of streptavidin (SA) enabled robust tetramer formation, supporting correct folding and utility for downstream immunological assays.

Need biotinylated pMHC production, refolding support, or tetramer assembly for T-cell assays? Talk to AtaGenix experts.

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HLA-I Expression and pMHC Tetramer Assembly in E. coli | AtaGenix

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