MTiterFast® Adjuvant – High-Performance Aqueous Adjuvant for Mice

ATO00072

MTiterFast® Adjuvant

Rapid Aqueous Adjuvant for Mice | pH-Neutral, Non-Toxic | 300+ Antibodies Developed

Product Description

MTiterFast® Adjuvant is a pH-neutral aqueous adjuvant with a bluish transparent appearance. Its primary component is a high-molecular-weight polymer whose hydrophobic ends bind to protein antigens (including peptides or small molecules conjugated to carrier proteins) without altering their structure. Once injected, the adjuvant slowly releases the bound antigen, gradually stimulating the immune system.

All auxiliary materials meet human-use pharmaceutical standards — non-toxic, non-immunogenic, and highly biocompatible. Contains no protein components, preventing antibody production against the adjuvant itself. MTiterFast® is primarily designed for use in mice.

Tested in 1,704 BALB/c mice with various antigen types (recombinant proteins, small molecules, peptides). Over 300 high-quality antibodies have been developed using MTiterFast®, validated in WB, IHC, IP, IF, Sandwich ELISA, indirect ELISA, and Neutralization assays.

Experimental Validation

MTiterFast Aqueous Adjuvant for Mice - Serum Titer Distribution

Fig 1. Serum titer distribution of 1,704 BALB/c mice immunized 3× with MTiterFast®. ~60% recombinant proteins, ~35% peptides, ~5% small molecules. Cutoff OD = 0.25.

Key Advantages

Proven Track Record 1,704 mice tested; 300+ high-quality antibodies developed across multiple applications.

Superior Safety Significantly reduced adverse reactions vs. Freund’s adjuvant. No inflammation or injection site necrosis.

Cost-Effective Reduces antigen consumption and shortens immunization cycles, lowering overall antibody production costs.

No Adjuvant Antibodies Protein-free formulation prevents antibody production against the adjuvant itself.

Ordering Information

Cat. No.	Product Name	Size	Price (USD)
ATO00072	MTiterFast Adjuvant (Rapid Adjuvant for Mice)	2 mL	$160
ATO00072	MTiterFast Adjuvant (Rapid Adjuvant for Mice)	5 mL	$360

Usage Guidelines

Immunization Protocol: Three immunizations recommended (Day 0, Day 7, Day 14). Collect serum on Day 21 for titer evaluation. If suboptimal, administer booster on Day 21–22.

Antigen Dosage:

Protein antigens / inactivated whole viruses / VLPs: 40–60 μg per mouse per dose, dilute to 100 μL, mix with 50 μL MTiterFast®.
Small molecules / peptides conjugated to carriers: 80–120 μg per mouse per dose, dilute to 100 μL, mix with 50 μL MTiterFast®.

Injection: 150 μL total per mouse. Recommended: two subcutaneous injections at the dorsal neck + one intraperitoneal injection, divided equally.

Preparation: Dilute antigen with sterile saline or PBS to recommended volume. Mix with adjuvant and inject immediately.

Storage: 4–8°C. Do not freeze. Shelf life: 2 years.

Precautions

Precipitation after mixing is normal. Mix thoroughly before loading syringe; inject immediately.
Optimized for mice; may have slightly reduced efficacy in other species.
Handle with care when opening to avoid injury from the aluminum seal.

Need adjuvants for mouse immunization? Explore our full TiterFast® series.

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For research use only.

Datasheet