IdeZ Protease

ATE00014

IdeZ Protease

Immunoglobulin G-degrading Enzyme | Recombinant, E. coli-expressed

Product Description

IdeZ Protease is a protease derived from Streptococcus equi subspecies zooepidemicus, known for its exceptional substrate specificity. It cleaves IgG at a specific site in the hinge region, generating intact F(ab')₂ fragments and Fc fragments. Compared to IdeS, IdeZ offers enhanced activity against mouse IgG2a and IgG3, making it a more versatile tool for antibody characterization across species.

Product Advantages

High Specificity Specifically cleaves IgG; compatible with human IgG1/2/3/4, monkey, sheep, rabbit, humanized, and chimeric IgGs.

Broad Compatibility Enhanced activity against mouse IgG2a and IgG3 compared to IdeS, enabling a wider range of preclinical applications.

Superior Performance Ideal for characterizing therapeutic antibodies, Fc fusion proteins, and antibody-drug conjugates (ADCs).

High Purity & Stability Recombinant E. coli expression; >95% purity by SDS-PAGE; animal-free production.

Applications

Therapeutic Antibody Characterization
Fc Fusion Protein Analysis
ADC Characterization
Antibody Drug Development

Product Specifications

Attribute	Details
Product Name	IdeZ Protease
Catalog Number	ATE00014
Host	E. coli
Tag	N-terminal His Tag
Molecular Weight	38 kDa
Form	Liquid
Buffer	PBS (pH 7.5), 50% glycerol
Purity	>95% (by SDS-PAGE)
Activity	50 U/μL
Reaction Conditions	37°C for 30 minutes
Storage	-20°C; shelf life 12 months
Shipping	Blue ice