rb-EK

ATE00002

Recombinant Bovine Enterokinase (rb-EK)

High-Efficiency Serine Protease for Fusion Tag Removal | Yeast-expressed

Product Description

Recombinant Bovine Enterokinase (rb-EK) is a highly specific serine protease that precisely recognizes the Asp-Asp-Asp-Asp-Lys (DDDDK) sequence and cleaves at the C-terminal side of Lys. It rapidly removes N-terminal fusion tags to release target proteins, making it an essential tool for protein purification and functional studies.

Product Advantages

High Specificity Accurately cleaves the DDDDK sequence, preventing nonspecific degradation of target proteins.

Exceptional Efficiency 1 mg rb-EK cleaves at least 1.5 g of fusion protein; suitable for large-scale production.

Broad Applicability Ideal for genetic engineering, biochemistry, molecular biology, and biopharmaceutical research.

Convenient Purification C-terminal His tag enables easy removal via metal affinity chromatography.

Applications

N-terminal Fusion Tag Removal
Protein Purification
Protein Functional Studies
Biopharmaceutical Development

Product Specifications

Attribute	Details
Product Name	Recombinant Bovine Enterokinase (rb-EK)
Catalog Number	ATE00002
Host	Yeast
Tag	C-terminal His Tag
Cleavage Site	DDDDK↓ (C-terminal side of Lys)
Molecular Weight	26.2 kDa
Form	Liquid
Buffer	50 mM Tris-HCl, 50% glycerol, pH 8.0
Purity	>95% (by SDS-PAGE)
Activity	2 U/μL
Specific Activity	>30 KU/mg
Unit Definition	Amount required to cleave 50 μg fusion protein in 16 hours at 25°C in 50 mM Tris-HCl (pH 8.0)
Reaction Conditions	25°C for 16 hours
Storage	-20°C; shelf life 12 months
Shipping	Blue ice

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