SUMO Protease

ATE00004

SUMO Protease

SUMO-specific Protease for Precise Tag Removal | Recombinant, E. coli-expressed

Product Description

SUMO Protease precisely recognizes SUMO-tagged proteins containing the 100-amino acid SUMO sequence and cleaves the bond between SUMO and the fusion protein. Its extended recognition sequence provides superior specificity compared to traditional EK or TEV proteases, significantly reducing nonspecific cleavage.

Product Advantages

Superior Specificity Extended recognition sequence ensures accurate SUMO tag cleavage even in complex samples.

Flexible Conditions Stable activity across 4–30°C and pH 7.0–9.0; works in both TBS and PBS buffers.

Convenient Purification His tag enables easy removal via affinity chromatography for high-purity target proteins.

High Purity Purity >95% by SDS-PAGE; recombinant E. coli expression ensures consistency.

Applications

SUMO Fusion Tag Removal
Target Protein Purification
Protein Structure & Functional Studies

Product Specifications

Attribute	Details
Product Name	SUMO Protease
Catalog Number	ATE00004
Host	E. coli
Tag	His Tag
Molecular Weight	26.55 kDa
Form	Liquid
Buffer	50 mM Tris-HCl pH 8.0, 150 mM NaCl, 50% glycerol
Purity	>95% (by SDS-PAGE)
Specific Activity	10 U/μL
Unit Definition	Amount required to cleave >85% of 2 μg substrate in 1 hour at 30°C
Reaction Conditions	pH 8.0; 4°C for 16 hours
Storage	-20°C; shelf life 12 months
Shipping	Blue ice