AtaGenix Laboratories
AtaGenix Laboratories
HRV 3C Protease | Recombinant, E. coli-expressed
Product Description
Rhinovirus 3C Protease, derived from human rhinovirus, specifically cleaves peptide bonds at the Gln-Gly site within the recognition sequence Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro (LEVLFQ↓GP). With high specificity and minimal nonspecific degradation, it is an efficient tool for removing fusion tags from recombinant proteins.
Product Advantages
Applications
Product Specifications
| Attribute | Details |
|---|---|
| Product Name | Rhinovirus 3C Protease |
| Catalog Number | ATE00003 |
| Specifications | 100 U / 500 U / 500 U × 2 |
| Host | E. coli |
| Tag | N-terminal GST Tag |
| Cleavage Site | LEVLFQ↓GP |
| Molecular Weight | 47.38 kDa |
| Form | Liquid |
| Buffer | 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 50% glycerol |
| Purity | >95% (by SDS-PAGE) |
| Activity | 500 U/mL |
| Specific Activity | 500 U/mg |
| Unit Definition | Amount required to cleave 100 μg substrate with >95% efficiency at 4°C for 16 hours |
| Reaction Conditions | 50 mM Tris-HCl (pH 7.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT; 4°C, 16 hours |
| Storage | -20°C; shelf life 12 months |
| Shipping | Blue ice |
Need 3C Protease or custom enzymatic tools for your protein research?
Request a QuoteFor research use only.
Contact Us
+86-27-6552-3339
info@atagenix.com
Building C, R & D Building, No. 666, Shendun 4th Road, Donghu New Technology Development Zone, Wuhan
