英文全称
Recombinant Human VEGFA/VEGF protein ,N- MAT Tag
纯度(Purity)
>90% as determined by SDS-PAGE
内毒素(Endotoxin level)
Please contact with the lab for this information.
蛋白构建(Construction)
A DNA sequence encoding the human VEGFA(Ala27~Arg136) was fused with the N-terminal MAT Tag
Accession #
P15692
表达宿主(Host)
E.coli
种属(Species)
Homo sapiens (Human)
预测分子量(Predicted Molecular Mass)
14.65kDa
制剂(Formulation)
Supplied as solution form in PBS, pH 7.5 8M urea or lyophilized from PBS, pH 7.5 8M urea.
运输方式(Shipping)
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性&储存(Stability &Storage)
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
复溶(Reconstitution)
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
背景介绍
VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.
分子别名(Alternative Names)
RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
参考文献(References)
Vanderstraeten, Baselet, Buset, Ben Said, de Ville de Goyet, Many, Gérard, Derradji (2020) Modulation of VEGF Expression and Oxidative Stress Response by Iodine Deficiency in Irradiated Cancerous and Non-Cancerous Breast Cells International journal of molecular sciences 21(11)