重组抗体被广泛地应用在科学研究、免疫诊断及治疗性抗体领域。普健生物推出AtaRATM保证型重组抗体表达服务。普健生物自有的哺乳表达系统高产量pATX系列载体及驯化293系列和CHO系列细胞，能实现全长抗体以及多种形式的重组抗体片段的高效分泌表达。无需免疫，可实现单克隆抗体及其它形式重组抗体的高产和可重复生产。

AtaRA^TM保证型重组抗体表达服务内容

1. 普健拥有抗体药物研发专家和技术支持团队，可对您感兴趣的抗体进行个性化的序列优化设计，提高重组抗体表达量。全长抗体分泌表达量可达100-300mg/L, 实现一次表达终身受益。

2. 丰富的抗体表达经验和技术团队，已经成功表达过多种形式的全长抗体（Human IgG1~4，Mouse IgG2b，Mouse IgG2a，Rabbit IgG等）抗体片段（scfv，Fab，tandem-scfv，VHH，Fc融合蛋白，bi-specificantibody等）。

3. 普健拥有多种服务平台，在实现重组抗体设计表达和纯化的同时，同时可以实现抗体的活性验证，目前已经设计完成了多个嵌合抗体的表达及活性验证，并对部分人鼠嵌合抗体进行人源化设计验证。真正实现一站式服务。

Recombinant Antibody Production Cases

Expression caseⅠ: scFv
Expression system：B.subtilis Expression System
Service Type：Recombinant Protein Production
Conclusion：Total obtained 37mg scFv from 1L scale up purification，purity is about 90%.

Figure 1. Expression result

Figure 2. Target scFv purification profile.

Figure 3. Final sample QC.

Expression caseⅡ: Bi-specific Antibody
Expression System ：Mammalian 293F Expression System
Service Type：Recombinant Antibody Production
Conclusion： Bi-specific antibody A total obtained 17.35mg per liter of culture，purity> 90%. Bi-specific antibody B total obtained 16.23mg per liter of culture，purity> 85%.

Figure 2. Target protein purification profile.

Figure 3. Final sample QC.

Expression caseⅢ：IgG-like bi-specific antibody
Expression System：Mammalian CHO-S Expression System
Service Type：Recombinant Antibody Production
Conclusion：Total obtained IgG-like: 104mg from 1L scale up purification, purity> 90%

Figure 1. Expression result .

Figure 2. Target protein purification profile.

Figure3. Final sample QC.

Expression caseⅢ：VHH expressed with tag and tag removal
Service Type：Recombinant Antibody Production
Conclusion：Total obtained VHH without any tag: 13.2mg from 1L scale up purification after tag removal, purity> 90%