重组抗体被广泛地应用在科学研究、免疫诊断及治疗性抗体领域。普健生物推出AtaRATM保证型重组抗体表达服务。普健生物自有的哺乳表达系统高产量pATX系列载体及驯化293系列和CHO系列细胞,能实现全长抗体以及多种形式的重组抗体片段的高效分泌表达。无需免疫,可实现单克隆抗体及其它形式重组抗体的高产和可重复生产。
AtaRATM保证型重组抗体表达服务内容
客户提供 | 抗体需求量 | 纯度/内毒素/proteinA/G残留检测 | 项目周期 | 交付内容 |
抗体序列 | 1~5mg | >95% by SDS-PAGE or HPLC Endotoxin<1EU/mg Concentration>1mg/L | 6~8周 | 基因合成的质粒 重组抗体 重组抗体表达报告 |
5mg~500mg | ||||
500mg~1g |
1. 普健拥有抗体药物研发专家和技术支持团队,可对您感兴趣的抗体进行个性化的序列优化设计,提高重组抗体表达量。全长抗体分泌表达量可达100-300mg/L, 实现一次表达终身受益。
2. 丰富的抗体表达经验和技术团队,已经成功表达过多种形式的全长抗体(Human IgG1~4,Mouse IgG2b,Mouse IgG2a,Rabbit IgG等)抗体片段(scfv,Fab,tandem-scfv,VHH,Fc融合蛋白,bi-specificantibody等)。
3. 普健拥有多种服务平台,在实现重组抗体设计表达和纯化的同时,同时可以实现抗体的活性验证,目前已经设计完成了多个嵌合抗体的表达及活性验证,并对部分人鼠嵌合抗体进行人源化设计验证。真正实现一站式服务。
Recombinant Antibody Production Cases
Expression caseⅠ: scFv
Expression system:B.subtilis Expression System
Service Type:Recombinant Protein Production
Conclusion:Total obtained 37mg scFv from 1L scale up purification,purity is about 90%.
Figure 1. Expression result
Figure 2. Target scFv purification profile.
Figure 3. Final sample QC.
Expression caseⅡ: Bi-specific Antibody
Expression System :Mammalian 293F Expression System
Service Type:Recombinant Antibody Production
Conclusion: Bi-specific antibody A total obtained 17.35mg per liter of culture,purity> 90%. Bi-specific antibody B total obtained 16.23mg per liter of culture,purity> 85%.
Figure 2. Target protein purification profile.
Figure 3. Final sample QC.
Expression caseⅢ:IgG-like bi-specific antibody
Expression System:Mammalian CHO-S Expression System
Service Type:Recombinant Antibody Production
Conclusion:Total obtained IgG-like: 104mg from 1L scale up purification, purity> 90%
Figure 1. Expression result .
Figure 2. Target protein purification profile.
Figure3. Final sample QC.
Expression caseⅢ:VHH expressed with tag and tag removal
Service Type:Recombinant Antibody Production
Conclusion:Total obtained VHH without any tag: 13.2mg from 1L scale up purification after tag removal, purity> 90%
Target protein purification profile in native condition.
Tag cleavage results.
1. Before cleavage sample 2. After cleavage sample
Final sample QC.