普健生物(武汉)科技有限公司(AtaGenix)
技术服务

重组抗体表达

重组抗体表达

  重组抗体被广泛地应用在科学研究、免疫诊断及治疗性抗体领域。普健生物推出AtaRATM保证型重组抗体表达服务。普健生物自有的哺乳表达系统高产量pATX系列载体及驯化293系列和CHO系列细胞,能实现全长抗体以及多种形式的重组抗体片段的高效分泌表达。无需免疫,可实现单克隆抗体及其它形式重组抗体的高产和可重复生产。

服务内容


AtaRATM保证型重组抗体表达服务内容

 

客户提供

抗体需求量

纯度/内毒素/proteinA/G残留检测

项目周期

交付内容

 

 

抗体序列

1~5mg

 

>95% by SDS-PAGE or HPLC

Endotoxin<1EU/mg

Concentration>1mg/L

 

 

6~8

基因合成的质粒

 

重组抗体

 

重组抗体表达报告

5mg~500mg

 

500mg~1g

服务流程


重组抗体表达流程

普健生物优势

1. 普健拥有抗体药物研发专家和技术支持团队,可对您感兴趣的抗体进行个性化的序列优化设计,提高重组抗体表达量。全长抗体分泌表达量可达100-300mg/L, 实现一次表达终身受益。

2. 丰富的抗体表达经验和技术团队,已经成功表达过多种形式的全长抗体(Human IgG1~4,Mouse IgG2b,Mouse IgG2a,Rabbit IgG等)抗体片段(scfv,Fab,tandem-scfv,VHH,Fc融合蛋白,bi-specificantibody等)。

3. 普健拥有多种服务平台,在实现重组抗体设计表达和纯化的同时,同时可以实现抗体的活性验证,目前已经设计完成了多个嵌合抗体的表达及活性验证,并对部分人鼠嵌合抗体进行人源化设计验证。真正实现一站式服务。

案例展示

Recombinant Antibody Production Cases

Expression caseⅠ: scFv
Expression system:B.subtilis Expression System
Service Type:Recombinant Protein Production
Conclusion:Total obtained 37mg scFv from 1L scale up purification,purity is about 90%.

重组抗体表达1

Figure 1. Expression result


重组抗体表达2

Figure 2. Target scFv purification profile.


重组抗体表达3

Figure 3. Final sample QC.


Expression caseⅡ: Bi-specific Antibody
Expression System :Mammalian 293F Expression System
Service Type:Recombinant Antibody Production
Conclusion: Bi-specific antibody A total obtained 17.35mg per liter of culture,purity> 90%. Bi-specific antibody B total obtained 16.23mg per liter of culture,purity> 85%.


重组抗体表达5

Figure 2. Target protein purification profile.


重组抗体表达6
Figure 3. Final sample QC.


Expression caseⅢ:IgG-like bi-specific antibody
Expression System:Mammalian CHO-S Expression System
Service Type:Recombinant Antibody Production
Conclusion:Total obtained IgG-like: 104mg from 1L scale up purification, purity> 90%


重组抗体表达7

Figure 1. Expression result .


重组抗体表达8

Figure 2. Target protein purification profile.


重组抗体表达9

Figure3. Final sample QC.


Expression caseⅢ:VHH expressed with tag and tag removal
Service Type:Recombinant Antibody Production
Conclusion:Total obtained VHH without any tag: 13.2mg from 1L scale up purification after tag removal, purity> 90%

重组抗体表达10
Target protein purification profile in native condition.

重组抗体表达11
Tag cleavage results.
1. Before cleavage sample 2. After cleavage sample

重组抗体表达12
Final sample QC.