普健生物(武汉)科技有限公司(AtaGenix)

Anti His tag mouse monoclonal antibody

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ATMA00001Mo
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产品名(Product Name)icon
Anti His tag mouse monoclonal antibody
货号(Catalog No.)icon
ATMA00001Mo
种类(Category)icon
Primary antibody
宿主(Host)icon
Mouse
反应种属(Species specificity)icon
Recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
应用实验(Tested applications)icon
WB,IP
克隆性(Clonality)icon
Monoclonal
克隆编号(Clone No.)icon
4E6
偶连物(Conjugation)icon
Unconjugated
免疫原(Immunogen)icon
Synthetic peptide HHHHHH-conjugated to KLH.
别名icon
6 His epitope tag,Hexa His tag,HHHHHH epitope tag,HHHHHH tag,His tag,Polyhistidine Tag.
Noteicon
For research use only .
状态(Form)icon
Liquid
储存溶液(Buffer)icon
Supplied as solution form in PBS, pH7.4, containing 0.05% proclin300, 50% glycerol.
存放条件(Storage)icon
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 4 °C for frequent use.
Store at -20 to -80 °C for twelve months from the date of receipt.
浓度(Concentration)icon
0.85 mg/ml
亚型(Isotype)icon
IgG1
纯化方式(Purity)icon
Protein G purification
WB: 1:1,000-5000,IP: 1:100-200
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

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