英文全称
Recombinant Human CTLA4 protein ,C- His Tag
纯度(Purity)
>90% as determined by SDS-PAGE
内毒素(Endotoxin level)
Please contact with the lab for this information.
蛋白构建(Construction)
A DNA sequence encoding the human CTLA4(Met1~Asp161) was fused with the C-terminal His Tag
Accession #
P16410
表达宿主(Host)
Mammalian cells
种属(Species)
Homo sapiens (Human)
预测分子量(Predicted Molecular Mass)
18.51kDa
制剂(Formulation)
Supplied as solution form in 150 mM NaCl, 50 mM Tris-HCl, pH 7.5 or lyophilized from 150 mM NaCl, 50 mM Tris-HCl, pH 7.5.
运输方式(Shipping)
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性&储存(Stability &Storage)
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
复溶(Reconstitution)
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
背景介绍
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
分子别名(Alternative Names)
CTLA4,CD152
参考文献(References)
Park, Shi, Kim, Jo, Cruz, Gou, Ha, Lu, Reich, Chen (2020) Force-dependent trans-endocytosis by breast cancer cells depletes costimulatory receptor CD80 and attenuates T cell activation Biosensors & bioelectronics 165() 112389