普健生物(武汉)科技有限公司(AtaGenix)

Recombinant Human TNF-alpha protein ,N- His Tag

  • 1
  • 2
ATMP00004HU
-
+
现货
加入购物车
收藏
  • 产品信息
  • 前沿进展(Frontier progress)
  • 如何订购
  • 说明书
概述(Summary)icon
英文全称
Recombinant Human TNF-alpha protein ,N- His Tag
纯度(Purity)
>90% as determined by SDS-PAGE
内毒素(Endotoxin level)
< 1.0 EU per μg of the protein as determined by the LAL method.
蛋白构建(Construction)
A DNA sequence encoding the human TNF(Val77~Leu233) was fused with the N-terminal His Tag
Accession #
P01375
表达宿主(Host)
Mammalian cells
种属(Species)
Homo sapiens (Human)
预测分子量(Predicted Molecular Mass)
17.32kDa
制剂(Formulation)
Supplied as solution form in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl or lyophilized from 50 mM Tris-HCl, pH 7.5, 150 mM NaCl.
运输方式(Shipping)
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性&储存(Stability &Storage)
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
复溶(Reconstitution)
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
电泳图(SDS-PAGE image)icon
    活性(Bioactivity)icon
      背景(Background)icon
      背景介绍
      Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
      分子别名(Alternative Names)
      DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
      参考文献(References)
      Reddy, Lakshmi, Maruthi Prasad, Varadacharyulu, Kodidhela (2020) Epigallocatechin gallate suppresses inflammation in human coronary artery endothelial cells by inhibiting NF-κB Life sciences () 118136
      The endothelium is a critical regulator of vascular homeostasis, controlling vascular tone and permeability as well as interactions of leukocytes and platelets with blood vessel walls. Consequently, endothelial dysfunction featuring inflammation and reduced vasodilation are considered central to cardiovascular disease (CVD) pathogenesis and have become a therapeutic area of focus. Type II endothelial cell (EC) activation by stress-related stimuli such as tumor necrosis factor-α (TNF-α) initiates the nuclear factor-κB (NF-κB) signaling pathway, a master regulator of inflammatory responses. Because dysregulated NF-κB signaling has been tightly linked to several CVDs, EC-specific inhibition of NF-κB represents an attractive pharmacological strategy. As accumulating evidence highlights the clinical benefits of tea catechin for multiple diseases including CVDs, we sought to determine whether the tea catechin epigallocatechin gallate (EGCG) that displays antioxidative, anti-inflammatory, hypolipidemic, anti-thrombogenic, and anti-hypertensive properties offers protection against CVDs by suppressing the canonical NF-κB pathway. Our findings indicate that EGCG downregulates multiple components of the TNF-α-induced NF-κB signaling pathway and thereby reduces the consequent increase in inflammatory gene transcription and protein expression. Furthermore, EGCG blocked type II EC activation, evidenced by diminished EC leakage and monocyte adhesion in EGCG-treated cells. In summary, our study advances knowledge of EGCG's anti-inflammatory effects on the NF-κB pathway and hence its benefits on endothelial health, supporting its therapeutic potential for CVDs.

      1搜索商品:关键字搜索;分类导航搜索;产品联系:手机/微信同号:15972188750,座机:027-65279366(推荐 !

      2产品选购:确定好产品信息与规格,向产品客服询问底价

      3提交订单:向客服提交收货人信息,选择送货方式,支付方式,发票信息

      4产品发货:发票随产品一起发送,耐心等待收货即可


      质量保证:

      所售产品均经过严格的质量检验,确保所供产品的技术指标与生产厂家的目录、说明书、质检报告上的描述一致,请放心选购。


      质量与免责说明:

      买方验收产品时,如发现产品短缺、不匹配、包装破损等情况,请保持产品原包装状态,并在收货七个工作日内,以书面方式向我司提出异议。逾期未提出异议视为合格。

      买方保管产品时,应按照产品标签、说明书所示保管条件保存,如系保管不当导致产品质量问题,恕不受理。

      买方使用产品时。如发现产品质量问题,应在使用初期提出,而非在使用完或快使用完时提出,以备我司回收产品,确认产品质量。如确属质量问题,我司负责调换或退货。发生索赔事宜,我司将在产品价格额度范围内酌情赔偿,恕不受理超出产品本身价值之部分。自收货之日起,超过三个月未反映产品问题,恕不退换。

      我司所提供的产品仅限于专业人士的科学研究使用,不得用于临床诊断或者治疗,若有单位或者个人擅自改变我司产品用途,我司不承担任何责任。