普健生物(武汉)科技有限公司(AtaGenix)

Recombinant Human TNF-alpha protein ,N- His Tag

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ATMP00004HU
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  • 产品信息(Product Details)
  • 前沿进展(Frontier progress)
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  • 说明书
概述(Summary)icon
英文全称
Recombinant Human TNF-alpha protein ,N- His Tag
纯度(Purity)
>90% as determined by SDS-PAGE
内毒素(Endotoxin level)
Please contact with the lab for this information.
蛋白构建(Construction)
A DNA sequence encoding the human TNF(Val77~Leu233) was fused with the N-terminal His Tag
Accession #
P01375
表达宿主(Host)
Mammalian cells
种属(Species)
Homo sapiens (Human)
预测分子量(Predicted Molecular Mass)
17.32kDa
制剂(Formulation)
Supplied as solution form in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl or lyophilized from 50 mM Tris-HCl, pH 7.5, 150 mM NaCl.
运输方式(Shipping)
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性&储存(Stability &Storage)
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
复溶(Reconstitution)
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
电泳图(SDS-PAGE image)icon
    活性(Bioactivity)icon
      背景(Background)icon
      背景介绍
      Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
      分子别名(Alternative Names)
      DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
      参考文献(References)
      Reddy, Lakshmi, Maruthi Prasad, Varadacharyulu, Kodidhela (2020) Epigallocatechin gallate suppresses inflammation in human coronary artery endothelial cells by inhibiting NF-κB Life sciences () 118136
      Noteicon
      For research use only .
      The endothelium is a critical regulator of vascular homeostasis, controlling vascular tone and permeability as well as interactions of leukocytes and platelets with blood vessel walls. Consequently, endothelial dysfunction featuring inflammation and reduced vasodilation are considered central to cardiovascular disease (CVD) pathogenesis and have become a therapeutic area of focus. Type II endothelial cell (EC) activation by stress-related stimuli such as tumor necrosis factor-α (TNF-α) initiates the nuclear factor-κB (NF-κB) signaling pathway, a master regulator of inflammatory responses. Because dysregulated NF-κB signaling has been tightly linked to several CVDs, EC-specific inhibition of NF-κB represents an attractive pharmacological strategy. As accumulating evidence highlights the clinical benefits of tea catechin for multiple diseases including CVDs, we sought to determine whether the tea catechin epigallocatechin gallate (EGCG) that displays antioxidative, anti-inflammatory, hypolipidemic, anti-thrombogenic, and anti-hypertensive properties offers protection against CVDs by suppressing the canonical NF-κB pathway. Our findings indicate that EGCG downregulates multiple components of the TNF-α-induced NF-κB signaling pathway and thereby reduces the consequent increase in inflammatory gene transcription and protein expression. Furthermore, EGCG blocked type II EC activation, evidenced by diminished EC leakage and monocyte adhesion in EGCG-treated cells. In summary, our study advances knowledge of EGCG's anti-inflammatory effects on the NF-κB pathway and hence its benefits on endothelial health, supporting its therapeutic potential for CVDs.

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